The structure of Tris is shown in the figure below, Tris is available in powder form and dissolved between pH 7.0 and 9.0. Tris is used in biochemistry, molecular biology and chemistry. The major role of the Tris in any biological practice is to maintain the pH of the solution and increase the permeability of the cell wall. Tris is a (hydroxymethyl)aminomethane having a molecular formula (HOCH 2) 3CNH 2. Separately, Tris and EDTA are used throughout the DNA extraction protocol as components of lysis buffer, elution buffer and washing buffer and helps to achieve our final goal that is to get the pure DNA. We need a pure DNA solution to use further. Usual DNA extraction protocols give output as precipitate which is impure and in solid form and therefore can’t be used for downstream applications. One such buffer is the TE (Tris- EDTA) buffer system. DNA extraction needs a specialized buffer system to protect the DNA from harmful chemical degradation.
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